Ficoll-Hypaque separation of bone marrow cells [letter]
نویسندگان
چکیده
منابع مشابه
Ficoll-Hypaque separation of bone marrow cells.
Levitt et aP and Greenberg et al’ have recently described elegant immunologic methodologies for selectively isolating T lymphocytes’ or hematopoietic progenitor cells2 from small aliquots of human marrow. In both cases the initial marrow manipulation, following aspiration, involved selective isolation of light-density mononuclear cells using Ficoll-Hypaque (I .077 ± 0.003 g/dL) gradients. Poten...
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A method is presented to separate turkey heterophils from anticoagulated whole blood using two-step Ficoll-Hypaque discontinuous gradients and ammonium chloride lysis of contaminating erythrocytes. Heterophils can be isolated from multiple blood samples within 3 to 4 hours. Using this technique, 66.4 +/- 18.4% (mean +/- standard deviation) of blood heterophils were harvested. Final cell isolate...
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Rapid separation of avian heterophils from anticoagulated whole blood was achieved using Ficoll-Hypaque discontinuous gradients. An average of 14.4% of blood heterophils was harvested with a mean purity exceeding 99%. Heterophil viability, as determined by trypan blue dye exclusion, averaged 99.8%. The integrity of isolated heterophils was evaluated by cytochemical staining and ultrastructural ...
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In the bone marrow, there are certain populations of stem cell sources with the capacity to differentiate into several different types of cells. Ideally, cell transplants would be readily obtainable, easy to expand and bank, and capable of surviving for sufficient periods of time. Bone marrow mesenchymal stem cells (BM-MSCs) possess all of these characteristics. One of the most important benefi...
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ژورنال
عنوان ژورنال: Blood
سال: 1985
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v66.2.472.bloodjournal662472